The ZN method requires the use of heat in the staining process while the K method does not. Combined with the rich color, this is a great plus. Negative Stain Negative stain uses negatively charged dye which can repel the negatively charged surfaces of bacteria resulting in the appearance of unstained bacteria cells with dark backgrounds.
To extend the service life of wood, the deck stain must have a number of important characteristics: However, the gram reaction does not ensure that the same antibacterial agent is able to destroy the 2 different species or strain of bacteria even though they showed the same result in the gram reaction.
Wood belongs to the category of soft materials, and all products used under the open sky require special protection. These three microorganisms appeared blue because of color of the dye, proving that the negative charges on their surfaces readily absorbed this positively charged basic stain.
Crystal violet dye can be replaced with Methylene blue. Steaming the bacterial emulsion on the slide while utilizing endospore staining procedure Stain conclusion Malachite green to enter the spore.
It comes in three colors: Both microorganisms remained unstained with dark backgrounds after staining with nigrosin. Smears from cultures of at least two microorganisms was prepared, one from each of the two groups.
Since this is an oil, you should be prepared for this factor anyhow. This indicates that both microorganisms were Gram-positive bacteria which have thicker peptidoglycans in their cell walls that trap the crystal violet-iodine complex more effectively, making them resistant to the decolorizer.
In the next step, safranin was used to counterstain both smears. Finally, we observed our specimen using microscope under oil-immersion objective lenses. This particular lens have more mirrors inside and it requires the use of oil to refract light rays towards the center of the lenses. Stains for treatment of indoor surfaces do not have the same degree of elasticity; therefore, the film soon flakes and starts peeling off.
The slide was briefly washed with tap water and the excess was drained off. In theory, both of this microorganisms are from two different groups, and we expected to observe two different result in this gram staining experiment.
Deck stain is used for treatment of the following structures: Mordant can be used to overcome this problem so that flagella will appear thick enough to be observed. The slides were examined under the oil-immersion objective.
Verdict We recommend this product if you want a clear stain for your deck, but you should try it out on an inconspicuous corner first and give it time to dry out before deciding to apply it all over. Simple Stain Table 3: This is because both are basic dye that have a positive charge that able to bind to negatively charged particle like teichoic acid at the bacterial cell wall.
However, take note that the color on the container may not quite be the color that you get when you apply it and it finally dries. When store shelves are filled with a vast variety of deck stains, you have to pay special attention to their selection and application.
Since this is an oil, you should be prepared for this factor anyhow.
Alcohol interacts with the lipid of cell membrane, causing gram negative cell lose its outer lipopolysaccharides LPS membrane, exposing the peptidoglycan layer. If moisture gets under the film, the wood will begin to deteriorate and the product will lose its authentic look.
This means that you can use it inside and out without concern for children or pets due to residue or runoff. These three microorganisms appeared blue because of color of the dye, proving that the negative charges on their surfaces readily absorbed this positively charged basic stain. The first type is used for surfaces that are constantly exposed to water.
The disadvantages of solvent-based deck stains include a long drying time about 24 hours and an ability to change the color of the wood — after application the wood acquires a yellowish color.
Usually, the drying time of applied deck stain depends on the base and takes about hours. Gram Staining Lab Report elleandrblog.com Free Download Here Lab #4: The Gram Stain Summary - University of Colorado Boulder Gram Stain -Conclusion 1. create a 4 frame cartoon that demonstrates your knowledge of Gram staining technique and HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY.
Conclusion Lab Unit 04 - Gram Stain Levine 1 Alexis Levine Maxwell T/R 7, February • The importance of the Gram stain. • Which organisms showed as Gram + and Gram -?
• What does the result of the Gram stain reveal about the organism? The purpose of the Gram stain is to separate bacteria into two different categories, Gram-positive 88%(8). Conclusion Lab Unit 04 - Gram Stain Levine 1 Alexis Levine Maxwell T/R 7, February • The importance of the Gram stain.
• Which organisms showed as Gram + and Gram -? • What does the result of the Gram stain reveal about the organism? The purpose of the Gram stain is to separate bacteria into two different categories, Gram-positive and Gram-negative based on chemical and physical 89%(9).
Mar 20, · Teeth are naturally white or off-white in color, but build-up from foods and drinks such as coffee and tea over time can stain the white color and turn teeth yellowish and discolored. In this experiment, we'll use egg shells in order to explore how teeth are stained by drinking coffee, tea, and cola.4/5(K).
Be sensitive to a color change in the area around the stain, otherwise this can be repeated until every trace of the stain is gone.
Peroxide is used by applying and waiting, sometimes repeatedly. Peroxide is a bleach, although a mild one, so test first on an inside seam.
Deck stain is designed to protect objects used outside from ultraviolet rays, pests, and high humidity. When store shelves are filled with a vast variety of deck stains, you have to pay special attention to their selection and application.Stain conclusion